Evaluation of antiparkinsonian activity of water yam tuber (Dioscorea alata L.) extract on haloperidol-induced Parkinson’s disease in mice

Parkinson’s disease (PD) appears as motor dysfunction that is attributed to depleting dopamine levels due cell death in the extrapyramidal system comprised of basal ganglia’s neurons. Oxidative stress central triggering death. Water yam (Dioscorea alata L.) contains anthocyanins with potential antioxidative and neuroprotective activities can ameliorate oxidative PD. This research aimed assess antiparkinsonian activity water yam’s ethanol extract by observing (bar test, rotarod negative geotaxis reflex cliff avoidance test) sensory (olfactory testing) vivo mice haloperidol-induced tubers were extracted maceration solvent 70% ethanol. experimental employed a posttest-only control group design where 35 equally distributed into 7 treatment groups, containing 5 each: Group 1 (normal group) was administered Carboxymethyl Cellulose Sodium (CMC-Na) orally (p.o.) aqua pro injection intraperitoneally (i.p.); 2 (negative given CMC-Na p.o.; Groups 3 4 (positive groups) received, respectively, levodopa p.o. curcumin then, finally, 5, 6, (test extracts at varying doses: 100, 200, 400 mg/kg BW. CMC-Na, levodopa, once day for days. Fifteen minutes after receiving their respective treatments on last day, all test animals, except 1, injected haloperidol solution BW i.p. Then, bar olfactory testing, performed examine responses 60, 120, 180th minutes. The data obtained inputted analyzed statistically One-Way ANOVA then continued an LSD test. In addition, thin-layer chromatography anthocyanin screening tubers. Results showed contained pelargonidin, when 200 mg/kg, it substantially shortened exploration time prolonged latency first fall tests relative haloperidol, groups. (p<0.05). Thus, be inferred pelargonidin (an compound) has preventing PD